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swescript rt ii first strand cdna synthesis kit  (Servicebio Inc)

 
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    Structured Review

    Servicebio Inc swescript rt ii first strand cdna synthesis kit
    Swescript Rt Ii First Strand Cdna Synthesis Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/swescript+rt+ii+first+strand+cdna+synthesis+kit/all+in+one+swescript/pm42284709-140-24-32
    Average 86 stars, based on 1 article reviews
    swescript rt ii first strand cdna synthesis kit - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: HIF1-driven TDP-43 stabilizes TRIP6 mRNA to drive angiogenesis and colorectal cancer progression under hypoxia.
    Article Snippet: Total RNA was isolated at 0, 4, 8, and 12 h post-treatment using TRIzol reagent (Invitrogen). .. RNA concentration and purity were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific). cDNA was synthesized from 1 μg of total RNA using the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio). .. Quantitative real-time PCR was performed using ArtiCanATM SYBR qPCR Mix (Tsingke Biotechnology) on an ABI QuantStudio6 Flex Real-Time PCR System.

    Spectrophotometry:

    Article Title: HIF1-driven TDP-43 stabilizes TRIP6 mRNA to drive angiogenesis and colorectal cancer progression under hypoxia.
    Article Snippet: Total RNA was isolated at 0, 4, 8, and 12 h post-treatment using TRIzol reagent (Invitrogen). .. RNA concentration and purity were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific). cDNA was synthesized from 1 μg of total RNA using the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio). .. Quantitative real-time PCR was performed using ArtiCanATM SYBR qPCR Mix (Tsingke Biotechnology) on an ABI QuantStudio6 Flex Real-Time PCR System.

    Synthesized:

    Article Title: HIF1-driven TDP-43 stabilizes TRIP6 mRNA to drive angiogenesis and colorectal cancer progression under hypoxia.
    Article Snippet: Total RNA was isolated at 0, 4, 8, and 12 h post-treatment using TRIzol reagent (Invitrogen). .. RNA concentration and purity were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific). cDNA was synthesized from 1 μg of total RNA using the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio). .. Quantitative real-time PCR was performed using ArtiCanATM SYBR qPCR Mix (Tsingke Biotechnology) on an ABI QuantStudio6 Flex Real-Time PCR System.

    Article Title: Naringenin, a Food-Derived Flavanone, Suppresses ITGA11-Associated Gastric Cancer Progression via the FAK/PI3K/AKT/mTOR Axis.
    Article Snippet: .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA employing the SweScript RT II First Strand cDNA Synthesis Kit (inclusive of gDNA Remover) (cat. no. G3333; Servicebio). .. Quantitative polymerase chain reaction (qPCR) was conducted using the 2 × Universal Blue SYBR Green qPCR Master Mix (with UDG) (cat. no. G3328, Servicebio) on a QuantStudio system.

    Article Title: HIF1-driven TDP-43 stabilizes TRIP6 mRNA to drive angiogenesis and colorectal cancer progression under hypoxia.
    Article Snippet: RNA concentration and purity were measured using a NanoDrop 2000C spectrophotometer (Thermo Scientific). .. Genomic DNA was removed and first-strand cDNA was synthesized from 1 μg of total RNA using the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio) with oligo (dT)18 primers. .. Quantitative real-time PCR was performed using the ArtiCanATM SYBR qPCR Mix (Tsingke Biotechnology) on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

    cDNA Synthesis:

    Article Title: HIF1-driven TDP-43 stabilizes TRIP6 mRNA to drive angiogenesis and colorectal cancer progression under hypoxia.
    Article Snippet: Total RNA was isolated at 0, 4, 8, and 12 h post-treatment using TRIzol reagent (Invitrogen). .. RNA concentration and purity were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific). cDNA was synthesized from 1 μg of total RNA using the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio). .. Quantitative real-time PCR was performed using ArtiCanATM SYBR qPCR Mix (Tsingke Biotechnology) on an ABI QuantStudio6 Flex Real-Time PCR System.

    Article Title: Naringenin, a Food-Derived Flavanone, Suppresses ITGA11-Associated Gastric Cancer Progression via the FAK/PI3K/AKT/mTOR Axis.
    Article Snippet: .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA employing the SweScript RT II First Strand cDNA Synthesis Kit (inclusive of gDNA Remover) (cat. no. G3333; Servicebio). .. Quantitative polymerase chain reaction (qPCR) was conducted using the 2 × Universal Blue SYBR Green qPCR Master Mix (with UDG) (cat. no. G3328, Servicebio) on a QuantStudio system.

    Article Title: DNA methylation-mediated silencing of MEOX1 promotes glycolysis and immune evasion in colorectal cancer cells through inhibition of GLP2R transcription.
    Article Snippet: .. Total RNA was extracted from cells or tissues using TRIzol (Invitrogen Inc., Carlsbad, CA, USA) and subjected to RT with SweScript RT II First Strand cDNA Synthesis Kit (With gDNA Remover) (G3333, Servicebio, Wuhan, Hubei, China). .. The Fast SYBR Green qPCR Master Mix (Low ROX) (G3324, Servicebio) was used for qPCR on an ABI 7900 HT Fast Real-Time Fluorescent Quantitative PCR System (Thermo Fisher).

    Article Title: ADAMTS9-AS2 acts as an epigenetic brake to constrain DNMT3B-mediated CADM2 silencing in esophageal squamous cell carcinoma metastasis
    Article Snippet: Genomic DNA contamination was eliminated by DNase I treatment (Thermo Scientific). .. First-strand cDNA synthesis was performed using 1 μg total RNA with the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio) in 20 μL reactions containing oligo(dT) primers and RT enzyme under thermal conditions of 25 °C for 5 min, 55 °C for 15 min, and 85 °C for 5 s. Quantitative PCR amplification was carried out using SYBR ® Select Master Mix (Applied Biosystems) on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). ..

    Article Title: USP15 stabilizes c-Myc to drive sunitinib resistance by suppressing cuproptosis in clear cell renal cell carcinoma.
    Article Snippet: RNA extraction and quantitative real-time polymerase reaction (qRT-PCR) The total RNA was extracted from cells and tissues using RNA extraction solution (Servicebio, Wuhan, China). .. SweScript RT II First Strand cDNA Synthesis Kit (Servicebio, Wuhan, China) was utilized to process reverse transcriptase reactions. .. We performed qRTPCR using the Roche LightCycler 480 detection system with 2×Universal Blue SYBR Green qPCR Master Mix (Servicebio, Wuhan, China).

    Article Title: HIF1-driven TDP-43 stabilizes TRIP6 mRNA to drive angiogenesis and colorectal cancer progression under hypoxia.
    Article Snippet: RNA concentration and purity were measured using a NanoDrop 2000C spectrophotometer (Thermo Scientific). .. Genomic DNA was removed and first-strand cDNA was synthesized from 1 μg of total RNA using the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio) with oligo (dT)18 primers. .. Quantitative real-time PCR was performed using the ArtiCanATM SYBR qPCR Mix (Tsingke Biotechnology) on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

    Article Title: Duplex DNase isothermal amplification-assisted magnetic nanoprobe platform with chemiluminescent and colorimetric readouts for microRNA and DNA detection
    Article Snippet: MicroRNAs (miRNAs) are non-coding RNAs that post-transcriptionally regulate cytoplasmic gene expression.. Among them, miRNA-375 (miR-375) has emerged as a promising biomarker for prostate cancer.. In this present study, we developed a Duplex DNase-assisted target recycling amplification strategy to detect miR-375 and HIV DNA fragments (target analytes).

    Article Title: Microplastics release from infusion sets during intravenous infusion induces cardiovascular toxicity.
    Article Snippet: .. Total RNA was then extracted using Trizol (Sigma, St. Louis, USA), followed by cDNA synthesis using the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio, Wuhan, China). qRTPCR was performed using the SYBR Green system, and data were analyzed by CT and 2-ΔΔCt methods. ..

    Real-time Polymerase Chain Reaction:

    Article Title: ADAMTS9-AS2 acts as an epigenetic brake to constrain DNMT3B-mediated CADM2 silencing in esophageal squamous cell carcinoma metastasis
    Article Snippet: Genomic DNA contamination was eliminated by DNase I treatment (Thermo Scientific). .. First-strand cDNA synthesis was performed using 1 μg total RNA with the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio) in 20 μL reactions containing oligo(dT) primers and RT enzyme under thermal conditions of 25 °C for 5 min, 55 °C for 15 min, and 85 °C for 5 s. Quantitative PCR amplification was carried out using SYBR ® Select Master Mix (Applied Biosystems) on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). ..

    Article Title: Duplex DNase isothermal amplification-assisted magnetic nanoprobe platform with chemiluminescent and colorimetric readouts for microRNA and DNA detection
    Article Snippet: MicroRNAs (miRNAs) are non-coding RNAs that post-transcriptionally regulate cytoplasmic gene expression.. Among them, miRNA-375 (miR-375) has emerged as a promising biomarker for prostate cancer.. In this present study, we developed a Duplex DNase-assisted target recycling amplification strategy to detect miR-375 and HIV DNA fragments (target analytes).

    Amplification:

    Article Title: ADAMTS9-AS2 acts as an epigenetic brake to constrain DNMT3B-mediated CADM2 silencing in esophageal squamous cell carcinoma metastasis
    Article Snippet: Genomic DNA contamination was eliminated by DNase I treatment (Thermo Scientific). .. First-strand cDNA synthesis was performed using 1 μg total RNA with the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio) in 20 μL reactions containing oligo(dT) primers and RT enzyme under thermal conditions of 25 °C for 5 min, 55 °C for 15 min, and 85 °C for 5 s. Quantitative PCR amplification was carried out using SYBR ® Select Master Mix (Applied Biosystems) on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). ..

    Reverse Transcription:

    Article Title: USP15 stabilizes c-Myc to drive sunitinib resistance by suppressing cuproptosis in clear cell renal cell carcinoma.
    Article Snippet: RNA extraction and quantitative real-time polymerase reaction (qRT-PCR) The total RNA was extracted from cells and tissues using RNA extraction solution (Servicebio, Wuhan, China). .. SweScript RT II First Strand cDNA Synthesis Kit (Servicebio, Wuhan, China) was utilized to process reverse transcriptase reactions. .. We performed qRTPCR using the Roche LightCycler 480 detection system with 2×Universal Blue SYBR Green qPCR Master Mix (Servicebio, Wuhan, China).

    SYBR Green Assay:

    Article Title: Duplex DNase isothermal amplification-assisted magnetic nanoprobe platform with chemiluminescent and colorimetric readouts for microRNA and DNA detection
    Article Snippet: MicroRNAs (miRNAs) are non-coding RNAs that post-transcriptionally regulate cytoplasmic gene expression.. Among them, miRNA-375 (miR-375) has emerged as a promising biomarker for prostate cancer.. In this present study, we developed a Duplex DNase-assisted target recycling amplification strategy to detect miR-375 and HIV DNA fragments (target analytes).

    Article Title: Microplastics release from infusion sets during intravenous infusion induces cardiovascular toxicity.
    Article Snippet: .. Total RNA was then extracted using Trizol (Sigma, St. Louis, USA), followed by cDNA synthesis using the SweScript RT II First Strand cDNA Synthesis Kit (Servicebio, Wuhan, China). qRTPCR was performed using the SYBR Green system, and data were analyzed by CT and 2-ΔΔCt methods. ..



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    swescript rt ii first strand cdna synthesis kit  (Servicebio Inc)
    86
    Servicebio Inc swescript rt ii first strand cdna synthesis kit
    Swescript Rt Ii First Strand Cdna Synthesis Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/swescript+rt+ii+first+strand+cdna+synthesis+kit/all+in+one+swescript/pm42284709-140-24-32
    Average 86 stars, based on 1 article reviews
    swescript rt ii first strand cdna synthesis kit - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

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